Director: Marshall Pope, PhD
Office : 355 EMRB
The Proteomics Core is staffed weekdays from 8 a.m. to 6 p.m. and is available 24/7 following training. A secure, web-based reservation system allows investigators to schedule instrument time.
The Proteomics Core assists researchers with micro-sequencing, structural analysis, and quantitative studies of proteins.
The Proteomics Core can assist with:
- Protein identification and mapping constituent peptides (rather advanced) for ligands, AA substitutions, and PTM
- Relative protein expression profiling (absolute quantification is also possible)
- Accurate weight determination for intact macromolecules and conjugates
- Development of quantitative MRM or PRM targeted assays (e.g. “multiplexed ELISA” with peptide standards rather than antibodies)
- Rapid (~15 sec/sample) biotyping to determine bacterial strains
- MASCOT, SEQUEST and SpectrumMill data analyses with final reporting in Scaffold or Excel format
- Training on Open Source versions of MaxQuant and Perseus (statistical package for “annotation enrichment” analysis)