Flow Cytometry Core
Director: Zuhair K. Ballas, MD
Office: 48 EMRB
The Flow Cytometry Facility is staffed weekdays from 8 a.m. to 6 p.m. and is available 24/7 following training. A secure, web-based reservation system allows investigators to schedule instrument time.
The 1,200-square foot facility is located in the Eckstein Medical Research Building (EMRB). The facility has one magnetic-based and 10 laser-based instruments whose major purpose is the identification and isolation of various cell populations. The laser-based instruments accomplish this by the use of antibodies to which various colors or dyes have been attached and are directed at molecules known to exist on the cell surface. By using several colors attached to different antibodies, one can identify and purify cells that express any given configuration of various molecules.
In addition to identification and isolation of various cell populations using antibodies, flow cytometry can analyze:
- Cell proliferation response to drugs or treatments, such as those used in chemotherapy
- Cell physiological properties, such as calcium flux and pH
- DNA content and integrity
- Transfection markers such as GFP and mCherry
Offline data analysis using FlowJoTM, DiVaTM, and ModFITTM can be done through the facility’s system of networked computers. Investigators can perform analysis at their laboratories or offices by accessing data through the facility’s file server. Data are archived on a separate server and stored for at least 10 years.
The facility provides scientific and technical personnel who are available for consultation in designing experimental protocols. Cell preparation protocols and publication quality output are available upon request.